Adaptation of latex-enhanced assay for percent glycohemoglobin to a Dade Dimension® analyzer
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منابع مشابه
Adaptation of latex-enhanced assay for percent glycohemoglobin to a Dade Dimension analyzer.
At present no method for glycohemoglobin (%HbA1c) is automated on a main-line analyzer to allow joint measurement with other indicators of diabetic control such as glucose and cholesterol. We describe an adaptation of a latex-enhanced competitive immunoassay for quantifying %HbA1c to the Dade International Dimension analyzer. After a manual hemolysis step, HbA1c and total hemoglobin (Hb) are de...
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This is an improved assay of C-reactive protein in serum, for use with the Baker "Encore" centrifugal analyzer. Features of this assay include: 250-specimen throughput per hour, within-batch CV 2.2%, between-batch CV 2.7%, no antigen-excess problems up to 1000 mg/L, negligible interference from rheumatoid factor, and good correlation (r = 0.99) with radial immunodiffusion. The method is inexpen...
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heterozygous, double-heterozygous and homozygous subjects, as established by 13 methods. Clin Chim Acta 1994;231:161–71. 10. Martina WV, Martijn EG, van der Molen M, Schermer JG, Muskiet FAJ. b-N-Terminal glycohemoglobin in subjects with common hemoglobinopathies: relation with fructosamine and mean erythrocyte age. Clin Chem 1993;39:2259–65. 11. Holownia P, Bishop E, Newman DJ, John GW, Price ...
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BACKGROUND Previous methods to measure glycohemoglobin (GHb) have been time-consuming or imprecise; we therefore developed a new enzymatic assay for GHb. METHODS Blood cells were first hemolyzed, and hemoglobin was digested with protease to yield fructosyl amino acid. Fructosyl amino acid oxidase acts on the fructosyl amino acid and generates hydrogen peroxide, which reacts with chromogens in...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1997
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/43.1.76